Construct: shRNA TRCN0000097857

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013668.2-764s1c1
DNA Barcode:
CGCATTGTTTATCCCTATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kdm5c (20591)
Gene Description:
lysine (K)-specific demethylase 5C
Transcript:
RefSeq NM_013668.2 (NON-CURRENT)
Match location:
Position 764 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097857)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCATTGTTTATCCCTATGAA
Hairpin Sequence:
5'-CCGG-CGCATTGTTTATCCCTATGAA-CTCGAG-TTCATAGGGATAAACAATGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCATTGTTTATCCCTATGAACTCGAGTTCATAGGGATAAACAATGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCATTGTTTATCCCTATGAACTCGAGTTCATAGGGATAAACAATGCG-3'

Other clones with same target sequence:

  1. TRCN0000287995