Construct: shRNA TRCN0000097900

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178280.3-4338s1c1
DNA Barcode:
CCCAACCTTAAATGAAGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sall3 (20689)
Gene Description:
sal-like 3 (Drosophila)
Transcript:
RefSeq NM_178280.3 (CURRENT)
Match location:
Position 4338 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000097900)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAACCTTAAATGAAGATTT
Hairpin Sequence:
5'-CCGG-CCCAACCTTAAATGAAGATTT-CTCGAG-AAATCTTCATTTAAGGTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAACCTTAAATGAAGATTTCTCGAGAAATCTTCATTTAAGGTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAACCTTAAATGAAGATTTCTCGAGAAATCTTCATTTAAGGTTGGG-3'

Other clones with same target sequence:

(none)