Construct: shRNA TRCN0000098089

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178870.2-1117s1c1
DNA Barcode:
CCTGGCCTTACTTCTGGACGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hs3st3a1 (15478)
Gene Description:
heparan sulfate (glucosamine) 3-O-sulfotransferase 3A1
Transcript:
RefSeq NM_178870.2 (NON-CURRENT)
Match location:
Position 1117 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000098089)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGCCTTACTTCTGGACGA
Hairpin Sequence:
5'-CCGG-CCTGGCCTTACTTCTGGACGA-CTCGAG-TCGTCCAGAAGTAAGGCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGCCTTACTTCTGGACGACTCGAGTCGTCCAGAAGTAAGGCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGCCTTACTTCTGGACGACTCGAGTCGTCCAGAAGTAAGGCCAGG-3'

Other clones with same target sequence:

(none)