Construct: shRNA TRCN0000098111

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152803.2-1520s1c1
DNA Barcode:
CGGATGGATTACTTTCCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hpse (15442)
Gene Description:
heparanase
Transcript:
RefSeq NM_152803.2 (NON-CURRENT)
Match location:
Position 1520 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000098111)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATGGATTACTTTCCAAAT
Hairpin Sequence:
5'-CCGG-CGGATGGATTACTTTCCAAAT-CTCGAG-ATTTGGAAAGTAATCCATCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATGGATTACTTTCCAAATCTCGAGATTTGGAAAGTAATCCATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATGGATTACTTTCCAAATCTCGAGATTTGGAAAGTAATCCATCCG-3'

Other clones with same target sequence:

(none)