Construct: shRNA TRCN0000098164

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133194.2-2241s1c1
DNA Barcode:
CCCTAAGAAGGGCATTACTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scml2 (107815)
Gene Description:
sex comb on midleg-like 2 (Drosophila)
Transcript:
RefSeq NM_133194.2 (NON-CURRENT)
Match location:
Position 2241 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000098164)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTAAGAAGGGCATTACTAT
Hairpin Sequence:
5'-CCGG-CCCTAAGAAGGGCATTACTAT-CTCGAG-ATAGTAATGCCCTTCTTAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTAAGAAGGGCATTACTATCTCGAGATAGTAATGCCCTTCTTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTAAGAAGGGCATTACTATCTCGAGATAGTAATGCCCTTCTTAGGG-3'

Other clones with same target sequence:

(none)