Construct: shRNA TRCN0000098183

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183178.1-545s1c1
DNA Barcode:
CGACAATATGAGTCACCTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fsd1 (240121)
Gene Description:
fibronectin type 3 and SPRY domain-containing protein
Transcript:
RefSeq NM_183178.1 (NON-CURRENT)
Match location:
Position 545 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000098183)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACAATATGAGTCACCTCAT
Hairpin Sequence:
5'-CCGG-CGACAATATGAGTCACCTCAT-CTCGAG-ATGAGGTGACTCATATTGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACAATATGAGTCACCTCATCTCGAGATGAGGTGACTCATATTGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACAATATGAGTCACCTCATCTCGAGATGAGGTGACTCATATTGTCG-3'

Other clones with same target sequence:

(none)