Construct: shRNA TRCN0000098287

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011600.2-1877s1c1
DNA Barcode:
CCTCCAAATCTAACAGGCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tle4 (21888)
Gene Description:
transducin-like enhancer of split 4
Transcript:
RefSeq NM_011600.2 (NON-CURRENT)
Match location:
Position 1877 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000098287)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCAAATCTAACAGGCATT
Hairpin Sequence:
5'-CCGG-CCTCCAAATCTAACAGGCATT-CTCGAG-AATGCCTGTTAGATTTGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCAAATCTAACAGGCATTCTCGAGAATGCCTGTTAGATTTGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCAAATCTAACAGGCATTCTCGAGAATGCCTGTTAGATTTGGAGG-3'

Other clones with same target sequence:

(none)