Construct: shRNA TRCN0000098294

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145925.1-303s1c1
DNA Barcode:
CTTCTCTCTGTAAATTGAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pttg1ip (108705)
Gene Description:
pituitary tumor-transforming 1 interacting protein
Transcript:
RefSeq NM_145925.1 (NON-CURRENT)
Match location:
Position 303 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000098294)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCTCTCTGTAAATTGAGTT
Hairpin Sequence:
5'-CCGG-CTTCTCTCTGTAAATTGAGTT-CTCGAG-AACTCAATTTACAGAGAGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCTCTCTGTAAATTGAGTTCTCGAGAACTCAATTTACAGAGAGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCTCTCTGTAAATTGAGTTCTCGAGAACTCAATTTACAGAGAGAAG-3'

Other clones with same target sequence:

  1. TRCN0000324536