Construct: shRNA TRCN0000098311

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019831.1-3887s1c1
DNA Barcode:
GCCTAGTTGTACTGGTCTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zmym3 (56364)
Gene Description:
zinc finger, MYM-type 3
Transcript:
RefSeq NM_019831.1 (NON-CURRENT)
Match location:
Position 3887 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000098311)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTAGTTGTACTGGTCTCAA
Hairpin Sequence:
5'-CCGG-GCCTAGTTGTACTGGTCTCAA-CTCGAG-TTGAGACCAGTACAACTAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTAGTTGTACTGGTCTCAACTCGAGTTGAGACCAGTACAACTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTAGTTGTACTGGTCTCAACTCGAGTTGAGACCAGTACAACTAGGC-3'

Other clones with same target sequence:

(none)