Construct: shRNA TRCN0000098315

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011370.1-3815s1c1
DNA Barcode:
CCAGTAACTGATGGCATGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cyfip1 (20430)
Gene Description:
cytoplasmic FMR1 interacting protein 1
Transcript:
RefSeq NM_011370.1 (NON-CURRENT)
Match location:
Position 3815 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000098315)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTAACTGATGGCATGTTT
Hairpin Sequence:
5'-CCGG-CCAGTAACTGATGGCATGTTT-CTCGAG-AAACATGCCATCAGTTACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTAACTGATGGCATGTTTCTCGAGAAACATGCCATCAGTTACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTAACTGATGGCATGTTTCTCGAGAAACATGCCATCAGTTACTGG-3'

Other clones with same target sequence:

  1. TRCN0000324539