Construct: shRNA TRCN0000098320

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025705.1-1751s1c1
DNA Barcode:
CCTCACTTACTGTTTACAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dcbld1 (66686)
Gene Description:
discoidin, CUB and LCCL domain containing 1
Transcript:
RefSeq NM_025705.1 (NON-CURRENT)
Match location:
Position 1751 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000098320)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCACTTACTGTTTACAGAA
Hairpin Sequence:
5'-CCGG-CCTCACTTACTGTTTACAGAA-CTCGAG-TTCTGTAAACAGTAAGTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCACTTACTGTTTACAGAACTCGAGTTCTGTAAACAGTAAGTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCACTTACTGTTTACAGAACTCGAGTTCTGTAAACAGTAAGTGAGG-3'

Other clones with same target sequence:

  1. TRCN0000324631