Construct: shRNA TRCN0000098337

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139001.1-1094s1c1
DNA Barcode:
GCATAGAAGATTTCAGTGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cspg4 (121021)
Gene Description:
chondroitin sulfate proteoglycan 4
Transcript:
RefSeq NM_139001.1 (NON-CURRENT)
Match location:
Position 1094 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000098337)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATAGAAGATTTCAGTGTTA
Hairpin Sequence:
5'-CCGG-GCATAGAAGATTTCAGTGTTA-CTCGAG-TAACACTGAAATCTTCTATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATAGAAGATTTCAGTGTTACTCGAGTAACACTGAAATCTTCTATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATAGAAGATTTCAGTGTTACTCGAGTAACACTGAAATCTTCTATGC-3'

Other clones with same target sequence:

(none)