Construct: shRNA TRCN0000098421

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010670.1-208s1c1
DNA Barcode:
CCTGCTATATGCATTCCTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Krtap12-1 (16694)
Gene Description:
keratin associated protein 12-1
Transcript:
RefSeq NM_010670.1 (CURRENT)
Match location:
Position 208 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000098421)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCTATATGCATTCCTGTT
Hairpin Sequence:
5'-CCGG-CCTGCTATATGCATTCCTGTT-CTCGAG-AACAGGAATGCATATAGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCTATATGCATTCCTGTTCTCGAGAACAGGAATGCATATAGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCTATATGCATTCCTGTTCTCGAGAACAGGAATGCATATAGCAGG-3'

Other clones with same target sequence:

(none)