Construct: shRNA TRCN0000098484

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030004.2-321s1c1
DNA Barcode:
CCAGAGAACCTAGAACTGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cryl1 (68631)
Gene Description:
crystallin, lambda 1
Transcript:
RefSeq NM_030004.2 (NON-CURRENT)
Match location:
Position 321 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000098484)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAGAACCTAGAACTGAAA
Hairpin Sequence:
5'-CCGG-CCAGAGAACCTAGAACTGAAA-CTCGAG-TTTCAGTTCTAGGTTCTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAGAACCTAGAACTGAAACTCGAGTTTCAGTTCTAGGTTCTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAGAACCTAGAACTGAAACTCGAGTTTCAGTTCTAGGTTCTCTGG-3'

Other clones with same target sequence:

(none)