Construct: shRNA TRCN0000098526

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027402.2-556s1c1
DNA Barcode:
CGAGCCCAATAACAACAAGGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fndc5 (384061)
Gene Description:
fibronectin type III domain containing 5
Transcript:
RefSeq NM_027402.2 (NON-CURRENT)
Match location:
Position 556 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000098526)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGCCCAATAACAACAAGGA
Hairpin Sequence:
5'-CCGG-CGAGCCCAATAACAACAAGGA-CTCGAG-TCCTTGTTGTTATTGGGCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGCCCAATAACAACAAGGACTCGAGTCCTTGTTGTTATTGGGCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGCCCAATAACAACAAGGACTCGAGTCCTTGTTGTTATTGGGCTCG-3'

Other clones with same target sequence:

(none)