Construct: shRNA TRCN0000098658

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019639.2-1200s1c1
DNA Barcode:
TGACACCATAGAGAATGTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ubc (22190)
Gene Description:
ubiquitin C
Transcript:
RefSeq NM_019639.2 (NON-CURRENT)
Match location:
Position 1200 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000098658)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGACACCATAGAGAATGTCAA
Hairpin Sequence:
5'-CCGG-TGACACCATAGAGAATGTCAA-CTCGAG-TTGACATTCTCTATGGTGTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGACACCATAGAGAATGTCAACTCGAGTTGACATTCTCTATGGTGTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGACACCATAGAGAATGTCAACTCGAGTTGACATTCTCTATGGTGTCA-3'

Other clones with same target sequence:

  1. TRCN0000316601