Construct: shRNA TRCN0000098717

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172819.1-3437s1c1
DNA Barcode:
GCTTTCTGAATCTGGAAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dip2b (239667)
Gene Description:
disco interacting protein 2 homolog B
Transcript:
RefSeq NM_172819.1 (NON-CURRENT)
Match location:
Position 3437 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000098717)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTCTGAATCTGGAAAGAT
Hairpin Sequence:
5'-CCGG-GCTTTCTGAATCTGGAAAGAT-CTCGAG-ATCTTTCCAGATTCAGAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTCTGAATCTGGAAAGATCTCGAGATCTTTCCAGATTCAGAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTCTGAATCTGGAAAGATCTCGAGATCTTTCCAGATTCAGAAAGC-3'

Other clones with same target sequence:

(none)