Construct: shRNA TRCN0000098844

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025380.1-322s1c1
DNA Barcode:
CTTGAAGATAAAGTCTACCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eef1e1 (66143)
Gene Description:
eukaryotic translation elongation factor 1 epsilon 1
Transcript:
RefSeq NM_025380.1 (NON-CURRENT)
Match location:
Position 322 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000098844)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGAAGATAAAGTCTACCTT
Hairpin Sequence:
5'-CCGG-CTTGAAGATAAAGTCTACCTT-CTCGAG-AAGGTAGACTTTATCTTCAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGAAGATAAAGTCTACCTTCTCGAGAAGGTAGACTTTATCTTCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGAAGATAAAGTCTACCTTCTCGAGAAGGTAGACTTTATCTTCAAG-3'

Other clones with same target sequence:

  1. TRCN0000308371