Construct: shRNA TRCN0000098928

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172683.1-3712s1c1
DNA Barcode:
CGCACTCACTTGTCAGAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pogz (229584)
Gene Description:
pogo transposable element with ZNF domain
Transcript:
RefSeq NM_172683.1 (NON-CURRENT)
Match location:
Position 3712 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000098928)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCACTCACTTGTCAGAAGAA
Hairpin Sequence:
5'-CCGG-CGCACTCACTTGTCAGAAGAA-CTCGAG-TTCTTCTGACAAGTGAGTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCACTCACTTGTCAGAAGAACTCGAGTTCTTCTGACAAGTGAGTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCACTCACTTGTCAGAAGAACTCGAGTTCTTCTGACAAGTGAGTGCG-3'

Other clones with same target sequence:

(none)