Construct: shRNA TRCN0000098957

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015788.1-6095s1c1
DNA Barcode:
CCTCTGTAATTGACAGTGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sacs (50720)
Gene Description:
sacsin
Transcript:
RefSeq NM_015788.1 (NON-CURRENT)
Match location:
Position 6095 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000098957)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTGTAATTGACAGTGTTA
Hairpin Sequence:
5'-CCGG-CCTCTGTAATTGACAGTGTTA-CTCGAG-TAACACTGTCAATTACAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTGTAATTGACAGTGTTACTCGAGTAACACTGTCAATTACAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTGTAATTGACAGTGTTACTCGAGTAACACTGTCAATTACAGAGG-3'

Other clones with same target sequence:

(none)