Construct: shRNA TRCN0000098967

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172661.2-3684s1c1
DNA Barcode:
CGCAGACCTCACACTAAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prrc2b (227723)
Gene Description:
proline-rich coiled-coil 2B
Transcript:
RefSeq NM_172661.2 (NON-CURRENT)
Match location:
Position 3684 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000098967)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAGACCTCACACTAAAGAT
Hairpin Sequence:
5'-CCGG-CGCAGACCTCACACTAAAGAT-CTCGAG-ATCTTTAGTGTGAGGTCTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAGACCTCACACTAAAGATCTCGAGATCTTTAGTGTGAGGTCTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAGACCTCACACTAAAGATCTCGAGATCTTTAGTGTGAGGTCTGCG-3'

Other clones with same target sequence:

(none)