Construct: shRNA TRCN0000098969

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172661.2-2337s1c1
DNA Barcode:
GCATCCTTCAGGACTGATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prrc2b (227723)
Gene Description:
proline-rich coiled-coil 2B
Transcript:
RefSeq NM_172661.2 (NON-CURRENT)
Match location:
Position 2337 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000098969)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCCTTCAGGACTGATGAA
Hairpin Sequence:
5'-CCGG-GCATCCTTCAGGACTGATGAA-CTCGAG-TTCATCAGTCCTGAAGGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCCTTCAGGACTGATGAACTCGAGTTCATCAGTCCTGAAGGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCCTTCAGGACTGATGAACTCGAGTTCATCAGTCCTGAAGGATGC-3'

Other clones with same target sequence:

(none)