Construct: shRNA TRCN0000099022

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177703.2-1248s1c1
DNA Barcode:
CCACTGCTTAGGAGGTGTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fbxw19 (235612)
Gene Description:
F-box and WD-40 domain protein 19
Transcript:
RefSeq NM_177703.2 (NON-CURRENT)
Match location:
Position 1248 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099022)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTGCTTAGGAGGTGTTAT
Hairpin Sequence:
5'-CCGG-CCACTGCTTAGGAGGTGTTAT-CTCGAG-ATAACACCTCCTAAGCAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTGCTTAGGAGGTGTTATCTCGAGATAACACCTCCTAAGCAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTGCTTAGGAGGTGTTATCTCGAGATAACACCTCCTAAGCAGTGG-3'

Other clones with same target sequence:

(none)