Construct: shRNA TRCN0000099031

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018884.1-935s1c1
DNA Barcode:
GCAAAGACTTATCCCGAGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pdzrn3 (55983)
Gene Description:
PDZ domain containing RING finger 3
Transcript:
RefSeq NM_018884.1 (NON-CURRENT)
Match location:
Position 935 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099031)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAAGACTTATCCCGAGCAA
Hairpin Sequence:
5'-CCGG-GCAAAGACTTATCCCGAGCAA-CTCGAG-TTGCTCGGGATAAGTCTTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAAGACTTATCCCGAGCAACTCGAGTTGCTCGGGATAAGTCTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAAGACTTATCCCGAGCAACTCGAGTTGCTCGGGATAAGTCTTTGC-3'

Other clones with same target sequence:

  1. TRCN0000287893