Construct: shRNA TRCN0000099132

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_484007.1-936s1c1
DNA Barcode:
GCCTTGATGAGCTGTGCCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm12138 (432549)
Gene Description:
predicted gene 12138; aldo-keto reductase family 1, member B3 pseudogene
Transcript:
RefSeq XM_484007.1 (NON-CURRENT)
Match location:
Position 936 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099132)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTGATGAGCTGTGCCAAA
Hairpin Sequence:
5'-CCGG-GCCTTGATGAGCTGTGCCAAA-CTCGAG-TTTGGCACAGCTCATCAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTGATGAGCTGTGCCAAACTCGAGTTTGGCACAGCTCATCAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTGATGAGCTGTGCCAAACTCGAGTTTGGCACAGCTCATCAAGGC-3'

Other clones with same target sequence:

(none)