Construct: shRNA TRCN0000099149

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172291.1-1065s1c1
DNA Barcode:
CAGACATTAGTGGAGTCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Foxred1 (235169)
Gene Description:
FAD-dependent oxidoreductase domain containing 1
Transcript:
RefSeq NM_172291.1 (NON-CURRENT)
Match location:
Position 1065 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099149)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGACATTAGTGGAGTCTATT
Hairpin Sequence:
5'-CCGG-CAGACATTAGTGGAGTCTATT-CTCGAG-AATAGACTCCACTAATGTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGACATTAGTGGAGTCTATTCTCGAGAATAGACTCCACTAATGTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGACATTAGTGGAGTCTATTCTCGAGAATAGACTCCACTAATGTCTG-3'

Other clones with same target sequence:

  1. TRCN0000308912