Construct: shRNA TRCN0000099160

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145595.1-842s1c1
DNA Barcode:
CTCATCTATATGGGTGACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cbr4 (234309)
Gene Description:
carbonyl reductase 4
Transcript:
RefSeq NM_145595.1 (NON-CURRENT)
Match location:
Position 842 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099160)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCATCTATATGGGTGACATT
Hairpin Sequence:
5'-CCGG-CTCATCTATATGGGTGACATT-CTCGAG-AATGTCACCCATATAGATGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCATCTATATGGGTGACATTCTCGAGAATGTCACCCATATAGATGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCATCTATATGGGTGACATTCTCGAGAATGTCACCCATATAGATGAG-3'

Other clones with same target sequence:

  1. TRCN0000351786