Construct: shRNA TRCN0000099213

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022811.1-699s1c1
DNA Barcode:
CCGGAAGACGTGTATAGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Polr1e (64424)
Gene Description:
polymerase (RNA) I polypeptide E
Transcript:
RefSeq NM_022811.1 (NON-CURRENT)
Match location:
Position 699 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099213)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGAAGACGTGTATAGATTT
Hairpin Sequence:
5'-CCGG-CCGGAAGACGTGTATAGATTT-CTCGAG-AAATCTATACACGTCTTCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGAAGACGTGTATAGATTTCTCGAGAAATCTATACACGTCTTCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGAAGACGTGTATAGATTTCTCGAGAAATCTATACACGTCTTCCGG-3'

Other clones with same target sequence:

(none)