Construct: shRNA TRCN0000099275

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029282.1-3513s1c1
DNA Barcode:
GCTCTAAGAGTTTAACTCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tti1 (75425)
Gene Description:
TELO2 interacting protein 1
Transcript:
RefSeq NM_029282.1 (CURRENT)
Match location:
Position 3513 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099275)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTAAGAGTTTAACTCTTT
Hairpin Sequence:
5'-CCGG-GCTCTAAGAGTTTAACTCTTT-CTCGAG-AAAGAGTTAAACTCTTAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTAAGAGTTTAACTCTTTCTCGAGAAAGAGTTAAACTCTTAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTAAGAGTTTAACTCTTTCTCGAGAAAGAGTTAAACTCTTAGAGC-3'

Other clones with same target sequence:

  1. TRCN0000332267