Construct: shRNA TRCN0000099416

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021491.2-1813s1c1
DNA Barcode:
CCAAAGAATTGTTGGGTACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smpd3 (58994)
Gene Description:
sphingomyelin phosphodiesterase 3, neutral
Transcript:
RefSeq NM_021491.2 (NON-CURRENT)
Match location:
Position 1813 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099416)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAAGAATTGTTGGGTACAT
Hairpin Sequence:
5'-CCGG-CCAAAGAATTGTTGGGTACAT-CTCGAG-ATGTACCCAACAATTCTTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAAGAATTGTTGGGTACATCTCGAGATGTACCCAACAATTCTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAAGAATTGTTGGGTACATCTCGAGATGTACCCAACAATTCTTTGG-3'

Other clones with same target sequence:

(none)