Construct: shRNA TRCN0000099425

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177678.4-2553s1c1
DNA Barcode:
CCCGCTAATGCTCTGTGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ablim2 (231148)
Gene Description:
actin-binding LIM protein 2
Transcript:
RefSeq NM_177678.4 (NON-CURRENT)
Match location:
Position 2553 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099425)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGCTAATGCTCTGTGTATT
Hairpin Sequence:
5'-CCGG-CCCGCTAATGCTCTGTGTATT-CTCGAG-AATACACAGAGCATTAGCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGCTAATGCTCTGTGTATTCTCGAGAATACACAGAGCATTAGCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGCTAATGCTCTGTGTATTCTCGAGAATACACAGAGCATTAGCGGG-3'

Other clones with same target sequence:

(none)