Construct: shRNA TRCN0000099428

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177678.4-188s1c1
DNA Barcode:
CCTGTGTAATACCTGTGGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ablim2 (231148)
Gene Description:
actin-binding LIM protein 2
Transcript:
RefSeq NM_177678.4 (NON-CURRENT)
Match location:
Position 188 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099428)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGTAATACCTGTGGGAA
Hairpin Sequence:
5'-CCGG-CCTGTGTAATACCTGTGGGAA-CTCGAG-TTCCCACAGGTATTACACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGTAATACCTGTGGGAACTCGAGTTCCCACAGGTATTACACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGTAATACCTGTGGGAACTCGAGTTCCCACAGGTATTACACAGG-3'

Other clones with same target sequence:

(none)