Construct: shRNA TRCN0000099441

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183086.1-405s1c1
DNA Barcode:
GCTGCTAAAGAACTTGGCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mrps10 (64657)
Gene Description:
mitochondrial ribosomal protein S10
Transcript:
RefSeq NM_183086.1 (NON-CURRENT)
Match location:
Position 405 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099441)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTAAAGAACTTGGCATT
Hairpin Sequence:
5'-CCGG-GCTGCTAAAGAACTTGGCATT-CTCGAG-AATGCCAAGTTCTTTAGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTAAAGAACTTGGCATTCTCGAGAATGCCAAGTTCTTTAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTAAAGAACTTGGCATTCTCGAGAATGCCAAGTTCTTTAGCAGC-3'

Other clones with same target sequence:

(none)