Construct: shRNA TRCN0000099454

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027299.2-879s1c1
DNA Barcode:
TGCGGAAGATTGCACCTGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Degs2 (70059)
Gene Description:
delta(4)-desaturase, sphingolipid 2
Transcript:
RefSeq NM_027299.2 (NON-CURRENT)
Match location:
Position 879 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099454)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCGGAAGATTGCACCTGAAT
Hairpin Sequence:
5'-CCGG-TGCGGAAGATTGCACCTGAAT-CTCGAG-ATTCAGGTGCAATCTTCCGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCGGAAGATTGCACCTGAATCTCGAGATTCAGGTGCAATCTTCCGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCGGAAGATTGCACCTGAATCTCGAGATTCAGGTGCAATCTTCCGCA-3'

Other clones with same target sequence:

(none)