Construct: shRNA TRCN0000099461

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025878.1-325s1c1
DNA Barcode:
CGGGATCACAAGTTGCATGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mrps18b (66973)
Gene Description:
mitochondrial ribosomal protein S18B
Transcript:
RefSeq NM_025878.1 (CURRENT)
Match location:
Position 325 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099461)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGATCACAAGTTGCATGTT
Hairpin Sequence:
5'-CCGG-CGGGATCACAAGTTGCATGTT-CTCGAG-AACATGCAACTTGTGATCCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGATCACAAGTTGCATGTTCTCGAGAACATGCAACTTGTGATCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGATCACAAGTTGCATGTTCTCGAGAACATGCAACTTGTGATCCCG-3'

Other clones with same target sequence:

  1. TRCN0000309500