Construct: shRNA TRCN0000099523

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173424.1-1455s1c1
DNA Barcode:
GCTGGAATATCACATCCGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zbtb37 (240869)
Gene Description:
zinc finger and BTB domain containing 37
Transcript:
RefSeq NM_173424.1 (NON-CURRENT)
Match location:
Position 1455 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099523)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGAATATCACATCCGCAA
Hairpin Sequence:
5'-CCGG-GCTGGAATATCACATCCGCAA-CTCGAG-TTGCGGATGTGATATTCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGAATATCACATCCGCAACTCGAGTTGCGGATGTGATATTCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGAATATCACATCCGCAACTCGAGTTGCGGATGTGATATTCCAGC-3'

Other clones with same target sequence:

(none)