Construct: shRNA TRCN0000099635

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024255.1-1631s1c1
DNA Barcode:
CCCACTGTCATGTAAGGACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hsdl2 (72479)
Gene Description:
hydroxysteroid dehydrogenase like 2
Transcript:
RefSeq NM_024255.1 (NON-CURRENT)
Match location:
Position 1631 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099635)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACTGTCATGTAAGGACAT
Hairpin Sequence:
5'-CCGG-CCCACTGTCATGTAAGGACAT-CTCGAG-ATGTCCTTACATGACAGTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACTGTCATGTAAGGACATCTCGAGATGTCCTTACATGACAGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACTGTCATGTAAGGACATCTCGAGATGTCCTTACATGACAGTGGG-3'

Other clones with same target sequence:

(none)