Construct: shRNA TRCN0000099640

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028459.1-3006s1c1
DNA Barcode:
GCCTCTTTACAGTACACTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wasl (73178)
Gene Description:
Wiskott-Aldrich syndrome-like (human)
Transcript:
RefSeq NM_028459.1 (NON-CURRENT)
Match location:
Position 3006 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099640)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTTTACAGTACACTAAT
Hairpin Sequence:
5'-CCGG-GCCTCTTTACAGTACACTAAT-CTCGAG-ATTAGTGTACTGTAAAGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTTTACAGTACACTAATCTCGAGATTAGTGTACTGTAAAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTTTACAGTACACTAATCTCGAGATTAGTGTACTGTAAAGAGGC-3'

Other clones with same target sequence:

(none)