Construct: shRNA TRCN0000099679

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009834.1-1333s1c1
DNA Barcode:
GAACACAACAACGGTCCAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Noct (12457)
Gene Description:
nocturnin
Transcript:
RefSeq NM_009834.1 (NON-CURRENT)
Match location:
Position 1333 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099679)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACACAACAACGGTCCAGAT
Hairpin Sequence:
5'-CCGG-GAACACAACAACGGTCCAGAT-CTCGAG-ATCTGGACCGTTGTTGTGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACACAACAACGGTCCAGATCTCGAGATCTGGACCGTTGTTGTGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACACAACAACGGTCCAGATCTCGAGATCTGGACCGTTGTTGTGTTC-3'

Other clones with same target sequence:

(none)