Construct: shRNA TRCN0000099701

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145367.2-597s1c1
DNA Barcode:
GCTGCATGTTTCTCAAGGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Txndc5 (105245)
Gene Description:
thioredoxin domain containing 5
Transcript:
RefSeq NM_145367.2 (NON-CURRENT)
Match location:
Position 597 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099701)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCATGTTTCTCAAGGCAA
Hairpin Sequence:
5'-CCGG-GCTGCATGTTTCTCAAGGCAA-CTCGAG-TTGCCTTGAGAAACATGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCATGTTTCTCAAGGCAACTCGAGTTGCCTTGAGAAACATGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCATGTTTCTCAAGGCAACTCGAGTTGCCTTGAGAAACATGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000331627