Construct: shRNA TRCN0000099702

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145367.2-1272s1c1
DNA Barcode:
CCAGGCAAAGGATGAACTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Txndc5 (105245)
Gene Description:
thioredoxin domain containing 5
Transcript:
RefSeq NM_145367.2 (NON-CURRENT)
Match location:
Position 1272 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099702)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGCAAAGGATGAACTATA
Hairpin Sequence:
5'-CCGG-CCAGGCAAAGGATGAACTATA-CTCGAG-TATAGTTCATCCTTTGCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGCAAAGGATGAACTATACTCGAGTATAGTTCATCCTTTGCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGCAAAGGATGAACTATACTCGAGTATAGTTCATCCTTTGCCTGG-3'

Other clones with same target sequence:

  1. TRCN0000302659