Construct: shRNA TRCN0000099704

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145367.2-1246s1c1
DNA Barcode:
GACTCCTTACACAGCTTTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Txndc5 (105245)
Gene Description:
thioredoxin domain containing 5
Transcript:
RefSeq NM_145367.2 (NON-CURRENT)
Match location:
Position 1246 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099704)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTCCTTACACAGCTTTGTT
Hairpin Sequence:
5'-CCGG-GACTCCTTACACAGCTTTGTT-CTCGAG-AACAAAGCTGTGTAAGGAGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTCCTTACACAGCTTTGTTCTCGAGAACAAAGCTGTGTAAGGAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTCCTTACACAGCTTTGTTCTCGAGAACAAAGCTGTGTAAGGAGTC-3'

Other clones with same target sequence:

  1. TRCN0000302660