Construct: shRNA TRCN0000099762

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172463.3-3947s1c1
DNA Barcode:
GCAGTAACTGTGAGATCACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sned1 (208777)
Gene Description:
sushi, nidogen and EGF-like domains 1
Transcript:
RefSeq NM_172463.3 (NON-CURRENT)
Match location:
Position 3947 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099762)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTAACTGTGAGATCACAA
Hairpin Sequence:
5'-CCGG-GCAGTAACTGTGAGATCACAA-CTCGAG-TTGTGATCTCACAGTTACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTAACTGTGAGATCACAACTCGAGTTGTGATCTCACAGTTACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTAACTGTGAGATCACAACTCGAGTTGTGATCTCACAGTTACTGC-3'

Other clones with same target sequence:

(none)