Construct: shRNA TRCN0000099785

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175549.3-3019s1c1
DNA Barcode:
CCGGCATTTATAGCTGGCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Robo2 (268902)
Gene Description:
roundabout guidance receptor 2
Transcript:
RefSeq NM_175549.3 (NON-CURRENT)
Match location:
Position 3019 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099785)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGCATTTATAGCTGGCATT
Hairpin Sequence:
5'-CCGG-CCGGCATTTATAGCTGGCATT-CTCGAG-AATGCCAGCTATAAATGCCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGCATTTATAGCTGGCATTCTCGAGAATGCCAGCTATAAATGCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGCATTTATAGCTGGCATTCTCGAGAATGCCAGCTATAAATGCCGG-3'

Other clones with same target sequence:

(none)