Construct: shRNA TRCN0000099814

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007530.2-328s1c1
DNA Barcode:
CCTTTATTCCTCCACAGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bcap29 (12033)
Gene Description:
B cell receptor associated protein 29
Transcript:
RefSeq NM_007530.2 (NON-CURRENT)
Match location:
Position 328 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099814)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTATTCCTCCACAGAGAT
Hairpin Sequence:
5'-CCGG-CCTTTATTCCTCCACAGAGAT-CTCGAG-ATCTCTGTGGAGGAATAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTATTCCTCCACAGAGATCTCGAGATCTCTGTGGAGGAATAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTATTCCTCCACAGAGATCTCGAGATCTCTGTGGAGGAATAAAGG-3'

Other clones with same target sequence:

  1. TRCN0000326785