Construct: shRNA TRCN0000099831

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133239.1-2466s1c1
DNA Barcode:
CCCAAGTTCATGGTAAACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Crb1 (170788)
Gene Description:
crumbs family member 1, photoreceptor morphogenesis associated
Transcript:
RefSeq NM_133239.1 (NON-CURRENT)
Match location:
Position 2466 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099831)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAGTTCATGGTAAACTTT
Hairpin Sequence:
5'-CCGG-CCCAAGTTCATGGTAAACTTT-CTCGAG-AAAGTTTACCATGAACTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAGTTCATGGTAAACTTTCTCGAGAAAGTTTACCATGAACTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAGTTCATGGTAAACTTTCTCGAGAAAGTTTACCATGAACTTGGG-3'

Other clones with same target sequence:

(none)