Construct: shRNA TRCN0000099936

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183222.2-1099s1c1
DNA Barcode:
CCTCAAATTCTCCTGGTATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fcrl5 (329693)
Gene Description:
Fc receptor-like 5
Transcript:
RefSeq NM_183222.2 (NON-CURRENT)
Match location:
Position 1099 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099936)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAAATTCTCCTGGTATAA
Hairpin Sequence:
5'-CCGG-CCTCAAATTCTCCTGGTATAA-CTCGAG-TTATACCAGGAGAATTTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAAATTCTCCTGGTATAACTCGAGTTATACCAGGAGAATTTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAAATTCTCCTGGTATAACTCGAGTTATACCAGGAGAATTTGAGG-3'

Other clones with same target sequence:

(none)