Construct: shRNA TRCN0000099965

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172050.1-1364s1c1
DNA Barcode:
GCAGAATAAATGTTTAGGGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cd300e (217306)
Gene Description:
CD300E molecule
Transcript:
RefSeq NM_172050.1 (NON-CURRENT)
Match location:
Position 1364 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000099965)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAATAAATGTTTAGGGTT
Hairpin Sequence:
5'-CCGG-GCAGAATAAATGTTTAGGGTT-CTCGAG-AACCCTAAACATTTATTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAATAAATGTTTAGGGTTCTCGAGAACCCTAAACATTTATTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAATAAATGTTTAGGGTTCTCGAGAACCCTAAACATTTATTCTGC-3'

Other clones with same target sequence:

(none)