Construct: shRNA TRCN0000100015
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_011248.1-4387s1c1
- DNA Barcode:
- CCCAAATGTTTCTTAACCCAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Robo3 (19649)
- Gene Description:
- roundabout guidance receptor 3
- Transcript:
- RefSeq NM_011248.1 (NON-CURRENT)
- Match location:
- Position 4387 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000100015)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCCAAATGTTTCTTAACCCAT
- Hairpin Sequence:
-
5'-CCGG-CCCAAATGTTTCTTAACCCAT-CTCGAG-ATGGGTTAAGAAACATTTGGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCCAAATGTTTCTTAACCCATCTCGAGATGGGTTAAGAAACATTTGGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCCAAATGTTTCTTAACCCATCTCGAGATGGGTTAAGAAACATTTGGG-3'
Other clones with same target sequence:
(none)
