Construct: shRNA TRCN0000100025

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177193.4-3213s1c1
DNA Barcode:
CCTTTCCTATAATGATCCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Islr2 (320563)
Gene Description:
immunoglobulin superfamily containing leucine-rich repeat 2
Transcript:
RefSeq NM_177193.4 (NON-CURRENT)
Match location:
Position 3213 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000100025)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTCCTATAATGATCCAAA
Hairpin Sequence:
5'-CCGG-CCTTTCCTATAATGATCCAAA-CTCGAG-TTTGGATCATTATAGGAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTCCTATAATGATCCAAACTCGAGTTTGGATCATTATAGGAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTCCTATAATGATCCAAACTCGAGTTTGGATCATTATAGGAAAGG-3'

Other clones with same target sequence:

(none)